Table 2. Summary of pilus phenotypes observed in derivatives of Lactococcus lactis NZ9000, Lactobacillus rhamnosus GG and PB12.
The AA replacement is shaded in light grey within the GYPSY motif (bold AA residues).
| Bacterial Derivatives | GYPSY Motif | Pili Assembly | Description of Pilus Phenotype |
|---|---|---|---|
| Lactobacillus rhamnosus GG | GFGVLCYPFASDAY | + | High molecular weight pili |
| Lactococcus lactis NZ9000+pAC44 | |||
| - wild-type SrtC1 | GFGVLCYPFASDAY | + | High molecular weight pili |
| - SrtC1Y29G, P30G | GFGVLCGGFASDAY | +/- | SpaA multimers |
| - SrtC1Y29G | GFGVLCGPFASDAY | + | High molecular weight pili |
| - SrtC1P30G | GFGVLCYGFASDAY | +/- | SpaA multimers |
| Lactobacillus rhamnosus PB12 | |||
| - negative control | n/a | - | No pili, SpaA monomers |
| - complemented with wild-type SrtC1 | GFGVLCYPFASDAY | + | High molecular weight pili |
| - complemented with SrtC1Y29G, P30G | GFGVLCGGFASDAY | +/- | SpaA multimers |
| - complemented with SrtC1Y29G | GFGVLCGPFASDAY | + | High molecular weight pili |
| - complemented with SrtC1P30G | GFGVLCYGFASDAY | +/- | SpaA multimers |
Legend: +, presence of pili; -, absence of pili; +/-, presence of short and/or rare pili; n/a, not applicable.