Table 3. Primers used for genetic analysis of Job’s tears accessions.
| Primer | Sequence | TA (°C) | NPF/NF | PPF (%) |
|---|---|---|---|---|
| UBC814 | (CT)8A | 54 | 8/8 | 100 |
| UBC824 | (TC)8G | 54 | 10/10 | 100 |
| UBC825 | (AC)8T | 54 | 8/11 | 72.7 |
| UBC826 | (AC)8C | 54 | 7/10 | 70.0 |
| UBC827 | (AC)8G | 52 | 7/10 | 70.0 |
| UBC842 | (GA)8YG | 52 | 9/10 | 90.0 |
| UBC857 | (AC)8YG | 55 | 7/9 | 77.8 |
| UBC874 | (CCCT)4 | 50 | 12/13 | 92.3 |
| UBC886 | VDV(CT)7 | 54 | 8/10 | 80.0 |
| UBC891 | HVH(TG)7 | 54 | 11/12 | 91.7 |
Y = (C, T); V = (A, C, G); D = (A, G, T); H = (A, C, T); TA: Annealing temperature; NPF: number of polymorphic fragments; NF: number of amplified fragments; PPF: percentage of polymorphic fragments.