Fig 5. Biological activity of compounds against infective Trypanosoma brucei brucei with downregulated expression of trypanothione synthetase (TryS).
A) Western blot analysis of cell extracts from 2x107 T. b. brucei from the wildtype (WT), 48 h tetracycline-induced (+) and non-induced (-) TryS-RNAi cell line. Two hundred ng of recombinant TbTryS was loaded as control. Bands from the molecular weight marker are indicated on left. The picture at the bottom shows the abundance of TryS for each condition as estimated by densitometry and expressed relative to the level of the WT cell line. B) Ponceau staining of the Western blot membrane that served as normalization control of protein load for each condition. C) Cytotoxicity (%) ± S.D. (n = 2) for tetracycline-induced (+) and non-induced (-) TryS-RNAi T. b. brucei treated with 5 μM nifurtimox or 100 nM EAP1-47.