Table 2. Features of LC and HN upon acidification in solution.
Features in solution | LC | HN |
---|---|---|
Trp1 | Exposed from pH 4.7 to 3.8 | Buried from pH 6.2 to 3.82 |
Secondary structure3 | Unchanged | Unchanged |
Tertiary structure3 | Increased | Unchanged |
Stability4 | Increased | Increased |
Influence over the other domain | ? | Stabilization |
1Trp fluorescence
2not shown
3CD
4GdnHCl denaturation