Table 2. Primer´s sequence used and extent of polymorphism.
| Marker | Primer | Primer´s Sequence (5´- 3´) | Total no. of loci/bands | No. of polymorphic loci/bands | % of Polymorphism (P) | Marker | Primer | Primer´s Sequence (5´- 3´) | Total no. of loci/bands | No. of polymorphic loci/bands | % of Polymorphism (P) |
|---|---|---|---|---|---|---|---|---|---|---|---|
| RAPD | OPA 02 | TGCCGAGCTG | 4 | 1 | 25.0 | ISSR | 01 | (CA)8RG | 5 | 4 | 80.0 |
| OPA 03 | AGTCAGCCAC | 6 | 0 | 0.0 | 03 | (GA)8YT | 9 | 9 | 100.0 | ||
| OPA 04 | AATCGGGCTG | 5 | 5 | 100.0 | 04 | (GA)8YC | 7 | 6 | 85.7 | ||
| OPA 05 | AGGGGTCTTG | 5 | 1 | 20.0 | 05 | (GA)8YG | 3 | 1 | 33.3 | ||
| OPA 07 | GAAACGGGTG | 1 | 0 | 0.0 | 06 | (AG)8YT | 5 | 5 | 100.0 | ||
| OPA 08 | GTGACGTAGG | 4 | 3 | 75.0 | 07 | (AG)8YC | 6 | 3 | 50.0 | ||
| OPA 09 | GGGTAACGCC | 4 | 3 | 75.0 | 09 | (AC)8YG | 1 | 0 | 0.0 | ||
| OPA 11 | CAATCGCCGT | 9 | 6 | 66.7 | 11 | (GT)8YG | 2 | 1 | 50.0 | ||
| OPA 13 | CAGCACCCAC | 6 | 2 | 33.3 | 12 | (AG)8YG | 5 | 1 | 20.0 | ||
| OPA 15 | TTCCGAACCC | 1 | 0 | 0.0 | 14 | (AG)8T | 5 | 5 | 100.0 | ||
| OPA 17 | GACCGCTTGT | 2 | 0 | 0.0 | 15 | (AG)8C | 6 | 3 | 50.0 | ||
| OPA 18 | AGGTGACCGT | 5 | 4 | 80.0 | 17 | (GA)8T | 6 | 5 | 83.3 | ||
| OPA 19 | CAAACGTCGG | 5 | 0 | 0.0 | 19 | (GA)8A | 3 | 2 | 66.7 | ||
| OPA 20 | GTTGCGATCC | 1 | 1 | 25.0 | 21 | (TC)8C | 1 | 0 | 0.0 | ||
| OPL 02 | TGGGCGTCAA | 1 | 0 | 0.0 | 23 | (CT)8RC | 2 | 1 | 50.0 | ||
| OPL 03 | CCAGCAGCTT | 6 | 3 | 75.0 | 25 | (TG)8RC | 1 | 0 | 0.0 | ||
| OPL 05 | ACGCAGGCAC | 3 | 0 | 0.0 | 27 | BDB(CA)7 | 9 | 3 | 33.3 | ||
| OPL 07 | AGGCGGGAAC | 3 | 0 | 0.0 | 28 | DBD(AC)7 | 6 | 1 | 16.7 | ||
| OPL 08 | AGCAGGTGGA | 4 | 3 | 75.0 | 29 | VHV(GT)7 | 3 | 1 | 33.3 | ||
| OPL 12 | GGGCGGTACT | 4 | 2 | 33.3 | 30 | HVH(TG)7 | 1 | 0 | 0.0 | ||
| OPL 14 | GTGACAGGCT | 2 | 0 | 0.0 | 31 | (AG)8VC | 2 | 1 | 50.0 | ||
| OPL 16 | AGGTTGCAGG | 2 | 0 | 0.0 | 32 | CCC(GT)7 | 3 | 2 | 66.7 | ||
| OPL 18 | ACCACCCACC | 4 | 2 | 33.3 | AFLP | ACC/CTT | 33 | 33 | 33.0 | ||
| OPN 12 | CACAGACACC | 2 | 0 | 0.0 | ACC/CAT | 29 | 25 | 25.0 | |||
| OPN 13 | AGCGTCACTC | 3 | 2 | 33.3 | ACC/CAG | 32 | 32 | 32.0 | |||
| OPN 15 | CAGCGACTGT | 5 | 2 | 33.3 | ACT/CAA | 6 | 2 | 2.0 |