Figure 1.

Representation of A. vulgare mtDNA map with confirmed expressed genes. Sense of the arrows indicates sense of transcription. Genes coding for proteins of the respiratory chain, for rRNAs and for tRNAs are represented by black, dark gray and gray arrows respectively. Below the map, images of ethidium bromide stained gels of RT-PCR products demonstrating the expression of the tRNA genes are presented. Experiments were designed with pairs of primers specific for each tRNA (Supplementary Table 1). The presence (+) or absence (−) of reverse transcriptase (RT) during the cDNA synthesis is indicated. Amino acids specifying tRNAs are represented by the one-letter code.