Figure 1.

RNA Affinity purified with NHP2 and SNU13 tagged proteins. (A)The specificity of the affinity selection by NHP2 and SUN13 tagged proteins. RNA from the beads (1/10 of the amount loaded was subjected to primer extension with a sequence-specific probe (Table S1), and separated on a 6% polyacrylamide denaturing gel. The identities of the tagged protein are indicated and the tested RNAs are listed below the image. (B) Purification of RNAs associated with NHP2 and SNU13. Purification was performed using 2×10¹¹ cells, as described in Materials and Methods. The purified RNPs were de-proteinized, and the RNA was separated on a 10% polyacrylamide-denaturing gel and stained with silver. The size of the pBR322 DNA-Msp I digest is indicated. Total RNA (1 μg) was used as a marker. Beads, the RNA extracted from the beads after the last step of purification.