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. 2015 Aug 14;12(10):1152–1158. doi: 10.1080/15476286.2015.1076612

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© 2015 The Author(s). Published with license by Taylor and Francis Group, LLC

This is an Open Access article distributed under the terms of the Creative Commons Attribution-Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. The moral rights of the named author(s) have been asserted.

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Figure 3. — Calibration of stable isotope labeled-internal standards (SIL-IS). (A) Various amounts of pure dm⁵C nucleoside were spiked with a constant molar amount (250 fmol) of chemically synthesized [D₃]dm⁵C. The slope of the double logarithmic plot of the ratio of the areas and the amount of nucleoside can be used to calculate the unknown amount of dm⁵C in the sample. (B) Calibration of S. cerevisiae SIL-IS, containing ¹³C labeled rm⁵C, was performed in the same manner. Instead of a constant molar amount of SIL-IS a constant mass of 50 ng was used.