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. 2015 Aug 14;12(10):1152–1158. doi: 10.1080/15476286.2015.1076612

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© 2015 The Author(s). Published with license by Taylor and Francis Group, LLC

This is an Open Access article distributed under the terms of the Creative Commons Attribution-Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. The moral rights of the named author(s) have been asserted.

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Figure 4. — Quantification results of dm⁵C and rm⁵C. (A) Ratios of dm⁵C/dC in commercial oligonucleotides from 6 different commercial suppliers. (B) Ratios of rm⁵C/rC in commercial oligonucleotides from 6 different commercial suppliers and one academic lab. (C) Absolute quantification of m⁵C per oligonucleotide on RNA and DNA of supplier 1, commercially supplied tRNA^Phe of S.cerevisae and RNA of supplier 5, as well as isolated tRNA^Met, E. coli as negative control. Oligonucleotides #47–50 contain m⁵C. The expected amount is indicated by the dark gray bars.