Table 1.

QQQ parameters of the dynamic MRM method

Modified nucleoside	Molecular Weight [Da]	Precursor ion [m/z]	Product ion [m/z]	Fragmentor Voltage [V]	Collision Energy [eV]	Cell accelerator voltage [V]	Retention time [min]
rm5C	257	258	126	40	9	2	9.0
rm5C 13C	267	268	131	40	9	2	9.0
dm5C	241	242	126	60	5	2	10.4
[D3]dm5C	244	245	129	60	5	2	10.7