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. 2016 Jan 19;13(2):172–176. doi: 10.1080/15476286.2015.1137420

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Figure 1. — Schematic of CRISPR/Cas9-mediated D-repeat knockout and vector construction. (A) Target site of CRISPR/Cas9-mediated mutagenesis in D-repeat of XIST gene. Gene structure of the human XIST with 6 exons represented as blank boxes and the tandem repeat of A-F are shown with boxes of different colors. The sequence of the sgRNA is shown according to the consensus of 7.6 D tandem repeat (TR) units. The PAM sequence (NGG) is shown in purple and the target sequence is shown in green. The q-PCR primers for the XIST are represented with a pair of red arrows. (B) The alignment of the sgRNA in TR1-TR8 is shown; the red means the mismatches sequence compared to the sgRNA. (C) Vector used in this study. The vector contains sgRNA insertion sites (pink) and the human codon optimized S. pyogenes Cas9 (hSpCas9) (orange).