Table 2.
Rsp5 alleles and their phenotypes
| Phenotype |
||||
|---|---|---|---|---|
| Mutant | rRNA | mRNA | tRNA | Reference |
| rsp5-1 | degradation of 25S rRNA | 53 | ||
| accumulation of poly(A)+ RNA in the nucleus | 71 | |||
| rsp5-19 | degradation of 18S and 25S rRNA (Northern and FISH analysis) | 53 | ||
| decreased total rate of ribosomes | 53 | |||
| reduced level of polysomes and 80S ribosomes | 53 | |||
| accumulation of poly(A)+ RNA in the nucleus | 90 | |||
| accumulation of tRNALeu precursors (Northern analysis) | 53 | |||
| accumulation of tRNATyr and tRNAMet in the nucleus (FISH analysis) | 90 | |||
| rsp5-3 | inhibition of nuclear export of the pre-60S subunit | 52 | ||
| accumulation of 35S pre-rRNA | 52 | |||
| decreased level of 20S and 27S pre-rRNAs | 52 | |||
| reduced level of mature 18S, 5.8S and 25S rRNAs | 52 | |||
| degradation of 18S and 25S rRNA | 53 | |||
| accumulation of poly(A)+ RNA in the nucleus | 52 | |||
| accumulation of tRNALeu and tRNATyr in the nucleus (FISH analysis) | 52 | |||
| accumulation of primary transcripts for tRNALeu and tRNAArg-Asp (Northern analysis) | 52 | |||
| rsp5-ww2 | accumulation of poly(A)+ RNA in the nucleus | 71 | ||
| rsp5-ww3 | accumulation of poly(A)+ RNA in the nucleus | 71 | ||
| rsp5-A401E | accumulation of HSF1 and MSN2/4 mRNAs in the nucleus | 72 | ||
| rsp5Δ | accumulation of poly(A)+ RNA in the nucleus | 71 | ||
| accumulation of mRNAs encoding Pgk1, Ssa1, Ssa2 and Ssa3 in the nucleus | 71 | |||