Table 1.
Kinetic parameters for eRF3a alleles and eRF3b interaction with PABP immobilized on a CM5 sensor chip surface.aa
| Protein | ka (M−1 s−1) | kd (s−1) | KD (nM) | Number of experiments | Foldb |
|---|---|---|---|---|---|
| eRF3a (10-GGC) | 1.61 +/− 0.38 × 105 | 2.12 +/− 0.26 × 10−4 | 1.5 +/− 0.2 | 5 | 1 |
| 12-GGC | 3.01 +/− 0.92 × 104 | 3.22 +/− 0.96 × 10−4 | 12.0 +/− 2.3 | 4 | 8 |
| 11-GGC | 1.31 +/− 0.13 × 105 | 3.74 +/− 0.23 × 10−4 | 2.9 +/− 0.4 | 3 | 2 |
| 7-GGC | 1.02 +/− 0.23 × 105 | 4.04 +/− 0.41 × 10−4 | 4.2 +/− 0.6 | 3 | 2.8 |
| eRF3b | 9.66 +/− 0.85 × 105 | 5.38 +/− 1.32 × 10−4 | 0.5 +/− 0.1 | 2 | 0.33 |
a
All differences in ka and KD between eRF3a 12-GGC allele and the other forms of eRF3 (7-GGC, 10-GGC, 11-GGC alleles and eRF3b) alleles were statistically significant (P < 0.05, unpaired Student's t-test), Values of kd, were not significantly different (P > 0.2). The SEM is indicated in the ka kd and KD columns.
b
Fold difference between eRF3a (10-GGC) and other alleles for KD values.