Table 2.

Kinetic parameters for eRF3a and eRF3a 12-GGC allele interaction with PABP bound to OligoA₁₂₀ immobilized on a SA sensor chip surface.^aa

Protein	ka (M−1 s−1)	kd (s−1)	KD (nM)	Number of experiments
eRF3a (10-GGC)	1.64 +/− 0.26 × 105	2.37 +/− 1.01 × 10−4	1.3 +/− 0.4	4
12-GGC	7.38 +/− 3.9 × 103	2.31 +/− 0.63 × 10−4	56.2 +/− 17.2	4

^aAll differences in k_a and K_D between eRF3a and the 12-GGC allele were statistically significant with P(k_a) = 0.00099 and P(K_D) = 0.01872 (unpaired Student's t-test), Values of k_d were not significantly different (P = 0.96). The SEM is indicated in the k_a, k_d and K_D columns.