Table 2.
Protein | ka (M−1 s−1) | kd (s−1) | KD (nM) | Number of experiments |
---|---|---|---|---|
eRF3a (10-GGC) | 1.64 +/− 0.26 × 105 | 2.37 +/− 1.01 × 10−4 | 1.3 +/− 0.4 | 4 |
12-GGC | 7.38 +/− 3.9 × 103 | 2.31 +/− 0.63 × 10−4 | 56.2 +/− 17.2 | 4 |
All differences in ka and KD between eRF3a and the 12-GGC allele were statistically significant with P(ka) = 0.00099 and P(KD) = 0.01872 (unpaired Student's t-test), Values of kd were not significantly different (P = 0.96). The SEM is indicated in the ka, kd and KD columns.