Figure 2.

The monomeric linear (+) and (−) RNAs of ELVd display a different mobility in non-denaturing PAGE. (A) Before electrophoresis, aliquots of the gel-eluted ELVd (+) and (−) forms (upper and lower panels, respectively) were heated at 98°C for 2 min and gradually-cooled at 35°C along 15 min (lane 2), snap-cooled on ice (lane 3), or applied directly with no thermal treatment (NT) (lane 4). Three other aliquots of the same RNAs were processed similarly, but in the presence of 5 mM Mg²⁺ (lanes 5, 6 and 7, respectively). (B) Aliquots of untreated ELVd (+) and (−) RNAs were applied individually (lanes 2 and 4, respectively) or concurrently (lane 3). Three other aliquots of the same RNAs were processed similarly, but in the presence of 5 mM Mg²⁺ (lanes 5, 6 and 7, respectively). M refers to DNA markers with their size (in base pairs) indicated on the left (lanes 1). Gels were stained with ethidium bromide and are shown in the inverted option to facilitate visualization.