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After CoCl2 treatment (200 μM, 4 h), the half-lives of VEGFA mRNA (A), as well as the half-lives of a control labile transcript (MYC mRNA) (B) and a control stable transcript (GAPDH mRNA) (C) were assessed by measuring the time required to achieve a 50% reduction in transcript levels after adding actinomycin D (Act.D). (D) The levels of VEGFA pre-mRNA following CoCl2 treatment were measured using intron-exon-spanning primers. **p < 0.01, Student's t-test, n = 4.
