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. Author manuscript; available in PMC: 2016 Apr 20.
Published in final edited form as: Curr Biol. 2015 Mar 26;25(8):1017–1030. doi: 10.1016/j.cub.2015.02.022

Figure 6. Root stem cell maintenance regulators establish early competence for shoot regeneration.

Figure 6

(A) Shoot regeneration in wild-type callus (Wild-type;PLT7::PLT1:YFP) derived from root explant after 12 days on SIM. (B,E) Competent calli turned green in plt3; plt5-2; plt7;PLT7::PLT1:vYFP root and leaf explants and in (C) plt3; plt5-2; plt7;PLT3::PLT2:GR root explants after 12 days on SIM. (D,F) Callus derived from root and leaf explants of plt3; plt5-2; plt7 remained yellowish on SIM. (G) Expression of PIN1-GFP in Wild-type; PIN1::PIN1:GFP after 7 days on CIM. (H) plt3; plt5-2; plt7;PLT7::PLT1:vYFP, PIN1::PIN1:GFP callus regained cellular morphology typical of wild-type and expressed PIN1-GFP after 7 days on CIM (I) Disorganized callus cells without PIN1-GFP expression in plt3; plt5-2; plt7;PIN1::PIN1:GFP on CIM. (J) Shoot progenitor cells labeled with PIN1-GFP in wild-type callus and (K) in plt3; plt5-2; plt7;PLT7::PLT1:vYFP, PIN1::PIN1:GFP after 7 days on SIM (L) No PIN1-GFP expression or shoot progenitor cell formation in plt3; plt5-2; plt7;PIN1::PIN1:GFP on SIM. Scale bar: 1mm in (A–F) and 50µm in (G–L). Red color in (G–I) is propidium iodide. No stain was used for cell boundaries in (J–L).