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. Author manuscript; available in PMC: 2016 Apr 12.
Published in final edited form as: Biochim Biophys Acta. 2014 Jul 10;1842(11):2060–2072. doi: 10.1016/j.bbadis.2014.07.005

FIGURE 6. NMR showed higher levels of majority of cytosolic HSPs than did mice (A, B). On the other hand, gel filtration fractions influencing PRS resistance contained only high levels of HSP72 and HSP40 (C).

FIGURE 6

(A) Multiplex Western blots of whole lysates and cytosolic preparations and their quantification (bar graphs) after standardization to total protein (Ponceau-S, see Figure S1) showed dramatic species differences in relative content of HSP90, HSP72, HSC70, HSP40, and HSP25. Mean values and standard error bars are shown (*p<0.005). (B) 5-hr SNs showed similar differences in HSPs content to that of the cytosolic preparations. (C) As demonstrated by Western blotting (left, molecular weights), SNNMR, fr.22, and fr.23 (underlined) contained high levels of HSP72, some HSP40, and virtually no HSP25. Arrows indicate elution of the molecular weight standards and numbers on the left of the figure represent molecular weights.