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. Author manuscript; available in PMC: 2016 Apr 12.
Published in final edited form as: Biochim Biophys Acta. 2014 Jul 10;1842(11):2060–2072. doi: 10.1016/j.bbadis.2014.07.005

FIGURE 8. HSP72 depletion affects not only inhibition resistance but also peptidase activity of proteasome.

FIGURE 8

(A) Up to 40% decline in ChT-L activity was observed when cytosolic lysates were mixed with at least anti-HSP70/72 antibodies (*p<0.001). (B) A similar decrease of activity with reconstituted naked-mole rat supernatant [SN] and partially purified naked mole-rat proteasomes [Prot] was observed (*p<0.001). (C) NMR proteasome [Prot] in the absence of SN does not exhibit inhibitor resistance (left). Moreover, NMR supernatant [SN] does not present any appreciable ChT-L peptidase activity (right).