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. Author manuscript; available in PMC: 2016 Apr 12.
Published in final edited form as: J Alzheimers Dis. 2015;47(4):1021–1033. doi: 10.3233/JAD-150242

Figure 2.

Figure 2

17MN functions upstream of RIPK1 in MC65 cells. A) Chemical structure of 17MN. B) U937 cells were pretreated with 17MN (1 or 3 μM) or Necrostatin-1 (10 μM) and pan caspase inhibitor zVAD (10 μM) for 1 h, then TNF-α (50 ng/mL) was added and incubated for 72 h. Cell viability was assessed by MTT assay. (**p < 0.01). C) MC65 cells were treated with 17MN (1 μM) under −TC conditions for 48 h. Medium was collected for analysis of extracellular Aβ40. Cells were lysed and analyzed for intracellular Aβ40. Total Aβ40 concentrations were normalized by total protein content of lysed cells. (*p < 0.05).