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. Author manuscript; available in PMC: 2016 Apr 12.
Published in final edited form as: J Alzheimers Dis. 2015;47(4):1021–1033. doi: 10.3233/JAD-150242

Figure 3.

Figure 3

The influence of 17MN on mitochondrial membrane potential. A) MC65 cells were treated with 17MN at indicated concentrations under −TC condition for 48 h. Cells were then incubated with TMRM (100 nM) for 30 min. Mean fluorescence intensity was measured by flow cytometry. (*p < 0.05 compared to −TC). B) SH-SY5Y cells were treated with 17MN (1 and 0.3 μM) and MPP+ (2.5 mM) for 24 h. Cells were then incubated with TMRM (100 nM) for 30 min. Mean fluorescence intensity was measured by flow cytometry. C and D) Oxygen consumption in mitochondria was measured using a Clark-type oxygen electrode at 30 °C using glutamate/malate (C) and using succinate/rotenone (D) in the presence or absence of 17MN.