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17MN-NBD localized to the mitochondria and ER of MC65 cells. A) Chemical structure of 17MN-NBD. B) MC65 cells were treated with 17MN or 17MN-NBD at indicated concentrations under −TC condition for 72 h. Cell viability was assessed by MTT assay. C and D) MC65 cells grown on cover slides were treated with 17MN-NBD (3 μM) and Mitotracker Red (300 nM) (C) or ERtracker Red (1 μM) (D). Cells were fixed with 4% formaldehyde and visualized by LSM710 confocal microscopy.
