Figure 1. The plasmid constructs used in this study.
(A) The constructs used in the FRET experiments. The full-length receptors had fluorescent proteins attached to their C-termini via a flexible GGS linker. The truncated receptors had the intracellular domain substituted with a fluorescent protein, which was attached to the TM domain via a longer flexible (GGS)5 linker. SP: signal peptide, EC: extracellular domain, TM: transmembrane domain, IC: Intracellular domain of VEGFR-2. Fluorescent protein was either YFP or mCherry. Amino acid residue numbers are shown above the constructs. (B) The constructs used in the Western blotting experiments.