Table 1.

Dimerization free energies, ΔG, and Intrinsic FRET efficiencies $\stackrel{\mathit{~}}{\mathit{E}}$, obtained from least-square parameter fits to the single-vesicle FRET data for the different VEGFR-2 constructs studied here. d is the distance between the fluorescent proteins in the dimers, calculated from the the Intrinsic FRET values under the assumption of free fluorescent protein rotation. The uncertainties are 67% confidence intervals (standard errors) calculated from the fit in Matlab.

DOI: http://dx.doi.org/10.7554/eLife.13876.008

VEGFR-2 variant	Dissociation constant, Kdiss(rec.μm-2)	△G (kcal.mol-1)	I-FRET	d (Å)
full length	35 (16 to 53)	-6.1 (-5.8 to -6.5)	0.82 (0.78 to 0.85)	41.3 (39.8 to 43)
EC+TM	3200 (2712 to 3750)	-3.4 (-3.3 to -3.5)	0.61 (0.55 to 0.68)	49.3 (46.8 to 51.4)
TM	310 (220 to 415)	-4.8 (-4.6 to -5.0)	0.61 (0.59 to 0.65)	49.3 (47.9 to 50.0)
EC+TM +VEGF-A121	100% dimer	100% dimer	0.45 ± 0.02	55 ± 1
EC+TM +VEGF-A165a	100% dimer	100% dimer	0.43 ± 0.02	56 ± 1
EC+TM +VEGF-A165b	100% dimer	100% dimer	0.42 ± 0.02	56 ± 1
EC+TM +VEGF-C	100% dimer	100% dimer	0.45 ± 0.02	55 ± 1
EC+TM +VEGF-D	100% dimer	100% dimer	0.43 ± 0.02	56 ± 1
EC+TM +VEGF-E	100% dimer	100% dimer	0.44 ± 0.02	55 ± 1
EC+TM(E764I-T771I-F778I)	100% dimer	100% dimer	0.31 ± 0.02	61 ± 1
EC+TM(E764I-T771I-F778I) + VEGF-A121	100% dimer	100% dimer	0.38 ± 0.02	58 ± 1
EC+TM(N762I-V769I-G770I)	3100 (2090 to 3600)	-3.4 (-3.3 to -3.7)	0.61 (0.58 to 0.65)	49.3 (47.9 to 50.3)
EC+TM(N762I-V769I-G770I) + VEGF-A121	100% dimer	100% dimer	0.24 ± 0.02	64 ± 1
EC(D4→D5) +TM	390 (271 to 480)	-4.7 (-4.5 to -4.9)	0.82 (0.78 to 0.89)	41.4 (37.5 to 43.0)
EC(D4→D5) + TM+VEGF-A121	210 (130 to 350)	-5.0 (-4.7 to 5.3)	0.83 (0.77 to 0.89)	41.0 (37.5 to 43.4)
EC(C482R)+TM	100% dimer	100% dimer	0.45 ± 0.02	55 ± 1
EC(C482R)+TM+VEGF-A121	100% dimer	100% dimer	0.47 ± 0.02	54 ± 1
EC(C482R)+TM(N762I-V769I-G770I)	100% dimer	100% dimer	0.31 ± 0.02	61 ± 1