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. Author manuscript; available in PMC: 2016 Apr 12.
Published in final edited form as: J Autoimmun. 2015 Feb 17;59:26–37. doi: 10.1016/j.jaut.2015.01.011

Figure 6.

Figure 6

Comparative analysis of cytokine secreting capacity between dnTGFβRII;Foxp3GFP mice Tregs and WT;Foxp3GFP mice derived Tregs.

Total mononuclear cells from spleen (A) and mesenteric lymph nodes (B) of dnTGFβRII;Foxp3GFP and WT;Foxp3GFP mice were stimulated with PMA and ionomycin for 3 hours in the presence of Golgi stop reagent. Secreted IFN-γ, IL-10 and Granzyme B (GzmB) of Tregs were assessed by flow cytometry. Graphs present 10 week-old mice, 4 mice per group. (C) and (D) present the statistics analysis of the data indicated in (A) and (B), respectively. Graphs present mean ± SD. Data is representative of two independent experiments with similar results. *P <0.05, **P <0.01 and ***P <0.001 as determined by Student Test.