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. Author manuscript; available in PMC: 2017 Apr 15.
Published in final edited form as: J Chromatogr B Analyt Technol Biomed Life Sci. 2016 Feb 8;1019:51–58. doi: 10.1016/j.jchromb.2016.02.009

Figure 4. Stability of thiols and disulfides in plasma.

Figure 4

Figure 4

Blood was treated with citrate buffer, plasma was then separated, treated again with citrate buffer, diluted with H2O and stored at −80°C for thiol analyses. Disulfides were measured on plasma-NEM obtained by RBC purification and stored at −80°C. At the indicated times protein thiols were measured by colorimetric conjugation with DTNB, LMM-SH (panel A), LMM-SS (panel B) and RSSP (panel C) were measured by HPLC analysis with fluorometric detection. n= 5.