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. Author manuscript; available in PMC: 2017 Apr 15.
Published in final edited form as: J Chromatogr B Analyt Technol Biomed Life Sci. 2016 Feb 8;1019:51–58. doi: 10.1016/j.jchromb.2016.02.009

Scheme 1. Suggested procedure for thiol and disulfide analysis in whole blood, red blood cells, plasma and platelets.

Scheme 1

Whole blood, red blood cell and platelet thiols and disulfides are stabilized by treatment of blood with NEM immediately after the collection. Red blood cells and platelets are then separated and either analyzed or stored at −80°C until analysis. The plasma samples obtained by purification of RBCs, and therefore already treated with NEM, are used for disulfide analyses. Plasma thiols are stabilized by treatment of whole blood with citrate buffer solution. Once plasma is separated, it can be either freshly measured or further diluted with H2O and citrate buffer before its storage at −80°C.