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. Author manuscript; available in PMC: 2017 Jun 1.
Published in final edited form as: Biochim Biophys Acta. 2016 Mar 11;1864(6):697–705. doi: 10.1016/j.bbapap.2016.03.008

Figure 2.

Figure 2

Effect of phospholipid polar headgroup and substrate aggregation state on LmPLC activity. (A) Relative activity of LmPLC towards different phospholipids (6 mM) dispersed in 20 mM Triton X-100, 20 mM HEPES, 150 mM NaCl pH 6, 37°C, compared to the % hydrolysis rate for PC (defined as 100). (B) Enzyme specific activity is shown as a function of diC7PC concentration in 20 mM MES, 150 mM NaCl, 50 μM Zn2+, 0.1 mg/mL BSA, pH 6. The black solid line is the fit for monomeric diC7PC as substrate; the dashed line is the fit for diC7PC micelles as substrates assuming a constant monomer activity and a sharp phase separation of monomers and micelles. Data are shown as mean ± SD, n = 3 independent experiments.