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. Author manuscript; available in PMC: 2017 Jun 1.
Published in final edited form as: Biochim Biophys Acta. 2016 Mar 11;1864(6):697–705. doi: 10.1016/j.bbapap.2016.03.008

Table 1.

ICP-MS analysis of LmPLC bound Zn2+ content under different conditions a.

sample Free Zn2+ (μM) b Total Zn2+ (μM) b PLC-Zn2+ (μM)b PLC (μM) c Zn2+/PLC
Non-Zn2+ buffer 0.040±0.003
LmPLC in non-Zn2+ buffer 0.040±0.003 1.30±0.02 1.26±0.02 2.97±0.15 0.42±0.02
Zn2+-buffer 5.04±0.06
LmPLC in Zn2+-buffer 5.04±0.06 8.73±0.07 3.69±0.07 1.78±0.09 2.1±0.2
a

Data are shown as mean ± SD, n = 3 independent experiments.

b

Free Zn2+ is the zinc concentration measured in the post-dialysis buffer sample; total Zn2+ is the concentration measured in the post-dialysis protein sample; PLC-Zn2+ is total Zn2+ less free Zn2+.

c

LmPLC protein concentration was measured with BCA protein assay [17].