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Kinetic parameters of LmPLC for substrates short-chain phosphatidylcholine substrates at pH 6 a.
| substrate | CMC (mM) b | Km (mM) | kcat (s−1) c | kcat/Km (mM−1 s−1) |
|---|---|---|---|---|
| diC4PC | 250 | 43.5±3.4 | 8.0±0.4 | 0.18±0.02 |
| diC6PC | 14 | 2.8±0.8 | 12.8±1.5 | 4.6±1.9 |
| diC7PC | 1.5 | 0.20±0.05 | 8.5±0.7 | 42±15 |
Data are shown as mean ± SD, n = 3 independent experiments.
CMC values for the short-chain PC molecules are from [33].
All assays were conducted in the presence of 50 μM ZnSO4.
