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. Author manuscript; available in PMC: 2017 Apr 15.
Published in final edited form as: J Chromatogr B Analyt Technol Biomed Life Sci. 2015 Dec 28;1019:202–209. doi: 10.1016/j.jchromb.2015.12.034

Fig. 6.

Fig. 6

Effect of GPx4 overexpression on ChOOH-induced chain peroxidation in tumor cells. A GPx4-overexpressing clone (6G4) of COH-BR1 cells and a vector control (VC) were labeled with [14C]Ch by incubating with a [14C]Ch-BSA complex (1:15 mol/mol). The labeling strength for both cell types was approximately the same. Labeled cells were incubated for 1 h or 2 h with non-peroxidized (control) or photoperoxidized DMPC/Ch (1.0/0.8 by mol) SUVs. The initial 7-OOH concentration in the latter system was ~150 μM. After incubation, cells were washed, harvested, and extracted; lipid fractions were analyzed by HPTLC-PI. (A) [14C]ChOX profiles. (B) Percent yields of various [14C]ChOX species. Means ± deviation of values from duplicate experiments are plotted. Reproduced from Ref. 21 with permission.