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. Author manuscript; available in PMC: 2017 Apr 15.
Published in final edited form as: Acta Biomater. 2016 Mar 2;35:32–41. doi: 10.1016/j.actbio.2016.03.001

Figure 6. Capillary networks with lumens for iPSC-ECs after 14 days of culture in PEG hydrogels that were polymerized within a microfluidic device.

Figure 6

CD31 (green), VE-Cadherin (red) and DAPI (nuclei, blue) expression for 1×107 iPSC-ECs/mL that were suspended in a 50% crosslinked PEG hydrogel and polymerized in the central channel of a tri-channel microfluidic device. Cells were cultured for 14 days in basal medium supplemented with 100 ng/mL VEGF. (A) A maximum intensity z-projection (45 µm thickness) in the central region of a microfluidic channel. (B) A 1 µm thick z-slice within the boxed region shown in A. Slice View images through the XZ and YZ planes along the dashed lines are shown to illustrate the organization through the thickness of the channel (45 µm thickness shown). (C) Zoomed image from region where dashed lines intersect in B illustrating lumen diameter for a larger capillary tubule. Scale bars: (A) 250 µm; (B) 100 µm.