Table 2. Results of sensitivity testing in feces.
| Type of spiked materials | Pathogens | LOD (/reaction) | Reproducibility CV (%) |
|---|---|---|---|
| Virus | Bovine viral diarrheal virus | 1.6 | 1.6 |
| (TCID50 or PFU) | Bovine enterovirus | 1.6 | 0.5 |
| Bovine torovirus | 1.6 | 0.6 | |
| Bovine adenovirus | 1.6 | 3.0 | |
| Bovine herpes virus-1 | 1.6 | 1.1 | |
| Bacteria | Salmonella Dublin | 13 | 1.7 |
| (CFU) | Salmonella Enteritidis | 1.3 | 2.8 |
| MAP | 1.3 | 1.3 | |
| Clostridium perfringens | 13 | 1.1 | |
| Enterotoxigenic Escherichia coli | 1.3 | 0.1 | |
| DNA | Mammalian orthoreovirus | 100 | 1.5 |
| (copy number) | *Bovine leukemia virus | 10.8 | 0.8 |
| Salmonella Typhimurium | 100 | 3.1 | |
| Eimeria zuernii | 100 | 5.2 | |
| Eimeria bovis | 100 | 0.2 | |
| Bovine coronavirus | 100 | 1.2 | |
| Group A rotavirus | 100 | 0.1 | |
| Group B rotavirus | 10 | 2.2 | |
| Group C rotavirus | 100 | 6.4 |
LOD: Limit of detection. CV: Coefficient value. MAP: Mycobacterium avium Spp. Paratuberculosis. *For bovine leukemia virus, the provirus was spiked into the feces.