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. 2015 Nov 21;78(3):405–410. doi: 10.1292/jvms.15-0598

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©2016 The Japanese Society of Veterinary Science

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Fig. 3. — Co-immunoprecipitation of USP9X with ICP0 in infected cells. (A-B) HEp-2 cells infected for 8 hr with wild-type HSV-1 (F) at MOI 5 were harvested (WCE, whole-cell extract) and immunoprecipitated (IP) with anti-Flag, anti-ICP0 (A) or anti-USP9X (B) antibody. Immunoprecipitates were analyzed by immunoblotting (IB) with indicated antibodies. (C) HeLa cells were infected for 8 hr with wild-type HSV-1 (F) or R7910 (ΔICP0) at MOI 5, harvested and immunoprecipitated with anti-ICP0 antibody. Immunoprecipitates were analyzed by immunoblotting with indicated antibodies.