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. 2016 Mar 20;7(6):656–663. doi: 10.7150/jca.13674

Figure 4.

Figure 4

HCA587/MAGE-C2 is involved in miR-874-regulated tumor cell proliferation and invasion. (A) MTS assays were performed at days 2 and 3 after transfection with si-HCA587/MAGE-C2 or si-NC. (B) Transwell invasion analysis of A375 cells treated with si-HCA587 or si-NC, and the quantitative analysis is shown in the right panel. (C) Cells counting analysis of A375 cells transfected with miR-874 or miR-NC plus HA-HCA587 expression vector or control at indicated times. (D) Western blot analyses of HCA587 expression in A375 cells transfected with miR-874 or miR-NC plus HA-HCA587 expression vector or control. (E) Transwell analysis of A375 cells treated with miR-874 or miR-NC plus HA-HCA587 expression vector or control, and the quantitative analysis is shown in the right panel. (mean ± S.D.; *** p < 0.001).