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Role of ALDH activity as a HNSCC TIC marker in sphere formation. (A) SAS cells sorted for ALDH positivity and plated for sphere formation. (B) Analysis of ALDH activity by flow cytometry and (C) Oct4 expression by Western blotting 24 h after the start of the primary sphere culture and 24 h after the start of the secondary sphere culture. Results show mean ± SD (n = 3; t-test; * P < 0.05).
