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. 2016 Apr 10;7(6):746–757. doi: 10.7150/jca.13289

Figure 1.

Figure 1

Highly expression of hERG1 in osteosarcoma cells and tissues. (A) Semi-quantitative RT-PCR was performed to measure hERG1 mRNA levels in SH-SY5Y, MG-63 and HEK293 cells. β-actin was used as the internal standard. (B) Protein expression of hERG1 was measured in SH-SY5Y, MG-63, and HEK293 cells by Western blot. GAPDH was used as the internal standard. (C) Immunohistochemistry staining for hERG1 in MG-63 cells. Images were captured using an Olympus light microscope equipped with a CCD color camera. (D) Immunohistochemistry of hERG1 in human brain (as positive control), osteosarcoma and osteosfibrous dysplasia samples. *** P < 0.001.