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. 2016 Apr 13;4:29. doi: 10.3389/fcell.2016.00029

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Copyright © 2016 te Winkel, Gray, Seistrup, Hamoen and Strahl.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Compatibility of DiSC₃(5) and GFP in microscopic single-cell experiments. (A) Phase contrast image (left panel) of cells expressing GFP-MinD (middle panel) and stained with DiSC₃(5) (right panel) in the absence and presence of gramicidin (5 μM). Note the strong decrease in DiSC₃(5) fluorescence and delocalization of MinD upon depolarization. (B) Phase contrast image (left panel) of cells expressing GFP-FtsZ (middle panel) and stained with DiSC₃(5) (right panel) in the absence and presence of gramicidin (5 μM). Note the reduced septal signal of FtsZ upon depolarization. Strains used: B. subtilis KS64 (GFP-MinD), and B. subtilis 2020 (GFP-FtsZ).