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Sequence and schematic representation of the F30-2xdBroccoli tag. (A) Sequence of F30-2xdBroccoli is presented. Green indicates the dBroccoli units, orange indicates the F30 sequence. An RNA of interest can be appended on either 5′ or 3′ end, or F30-2xdBroccoli can be inserted inside of an RNA of interest. (B) Schematic drawing of F30-2xdBroccoli. Two Broccoli units (green) are inserted into each of two stem-loops of F30 (orange).
