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F30-2xdBroccoli imaging in E. coli. The pET28c or pET28c-F30-2xdBroccoli plasmids were transformed into bacteria, then RNA was expressed for 4 h and the cells were processed as described in the Basic Protocol 3 and imaged. Broccoli fluorescence was detected in the FITC channel (excitation filter 470±20 nm and emission filter 525±25 nm). Exposure time 200 ms. Scale bar, 5 μm.
