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. Author manuscript; available in PMC: 2016 Oct 1.
Published in final edited form as: Expert Rev Hematol. 2015 Sep 1;8(5):669–679. doi: 10.1586/17474086.2015.1078235

Table 2.

Summary of Mechanisms of HbF production in response to hydroxyurea

Signal level Pathway Mechanism of γ-globin induction References
Epigenetic & Transcriptional Events DNA remodelling
DNA methylation
Mi-2/NuRD; Acetylation and methylation DNMT-1 [31,38,84,133,134]
[131, 132]

Signalling pathways Giα/JNK/Jun NFκB induction of SAR1 [82]

cGMP sGC-PKG and NO-induced sGC-PKG [86,88,90,91]

cAMP MYB transcriptional control [90,124,135]

MAPKs Erk1-2/p38/JNK/CREB1 [91,97100,136]

Nitric oxide eNOS, flow-induced ATP and anti-sickling [85,123]

Phosphorylation ElF2α [128]

Transcription factors GATA-1/2; p21; EPO; KLF1 [32,101,137]

Stress signals ROS (superoxide and hydroxyl radicals) [104]

Post-transcription Translational Regulation miR-494; 15a; 16-1; 151-3p; 148b [84,124126,138141]