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Altered IRS1 phosphorylation in the brain of the diabetic monkeys. Western blot analysis showing phospho-Ser616 and total protein levels for IRS1 in each of the four indicated brain regions. Quantitation of phospho-IRS1 normalized to the total protein level is given in the bottom four graphs for each region. β-Tubulin was used as a loading control. **p < 0.01.
