Figure 5.
Automated screening using cultured postnatal rat cerebellar granule neurons reveals that CD2AP is a positive regulator of axon outgrowth. A, Electroporation of expression plasmids for the indicated genes into CGNs reveals effects on axon outgrowth. *Statistically significant changes compared with GFP (ANOVA and post hoc t test). B, Reduction of CD2AP protein levels using four different shRNA plasmids (1–4) with differing efficiencies reduced outgrowth in accordance with CD2AP levels. Inset (over neurite-length bar graph), Example Western blot of CD2AP protein expression after transfection of PC12 cells with siRNA plasmids (1–4) or scrambled-sequence control (Sc.) plasmid. *Statistically significant changes compared with scrambled-sequence plasmid (ANOVA followed by post hoc t test).