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. 2016 Apr 13;36(15):4259–4275. doi: 10.1523/JNEUROSCI.2423-15.2016

Figure 6.

Figure 6.

NGF treatment upregulates CD2AP, which is a positive regulator of PC12 neurite growth and complexity. A, PC12 cells were treated with NGF for 24 h before harvesting and processing for Western blot analysis. Ai, Representative blot. Aii, Densitometric quantification. *Significant difference (Student's t test). The effect of increasing the levels of CD2AP on neurite morphology was examined with PC12 cells transfected with plasmids for GFP (control) or GFP-CD2AP fusion protein for 24 h and then treated with NGF for 48 h. Examples of neurite morphology are provided for GFP-transfected (B) and GFP-CD2AP-transfected (C) PC12 cells. Neurite morphology of transfected populations was characterized by Sholl analysis (D) and by total neurite length per cell (E). *Statistically significant difference between groups (repeated-measures ANOVA with post hoc t test).