Table 5. Calculation of the number of replicons per replication focus from the experimental data.
| Experimental data | Human HeLa Kyoto (Mean±s.e.m.) | Mouse C2C12 (Mean±s.e.m.) |
|---|---|---|
| RFS, 103 Ntd per min | 1.65±0.12 | 2.46±0.11 |
| IOD, kbp | 188.7±17.2 | 161.7 ±16.3 |
| GS, 103 Mbp | 9.7±0.002 | 11.4±0.006 |
| Active RFi at any given time point | 5,583±162 | 5,314±227 |
| Total S-phase duration, minutes | 570±9 | 564±23 |
| Calculations | Human HeLa Kyoto (Mean±MSE) | Mouse C2C12 (Mean±MSE) |
| Time to replicate the genome with one fork (GS/RFS), hours | 97,662±1.95 × 10−5 | 77,230±1.19*10−5 |
| Replication forks active in parallel (GS/RFS/S-phase duration) | 10,298±1 | 8,216±0.0 |
| Replicons active in parallel (active forks/2) | 5,149 | 4,108 |
| Replicons per RFi (calculated replicons active in parallel/counted RFi) | 0.92±0.2 | 0.77±0.3 |
GS, Genome size; IOD, Inter-origin distance; MSE, mean squared error; RFi, replication foci; RFS, replication fork speed.
See equation (3) in Materials and methods.