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. 2016 Mar 30;2016:8753436. doi: 10.1155/2016/8753436

Figure 8.

Figure 8

Effects of MB salt/M/B on glucose production. HepG2 cells were treated with different concentrations of MB salt (a) or an equal concentration of MB salt/M/B (b) for 24 h. Alternatively, cells were pretreated with CC for 30 min; then MB salt (c), M (d), or B (e) was added and incubated for 24 h. DMSO (0.1%) was used as control. After treatment, culture media were discarded; cells were loaded with the glucose production medium as described in Section 2. Four hours later, glucose levels in the supernatant were determined, normalized to protein concentrations, and presented as percentages of DMSO. Values are mean ± SD of 3 separate experiments; ∗ p < 0.05 and ∗∗ p < 0.01 versus that of DMSO; $ p < 0.05 versus that of M alone or B alone in (b); # p < 0.05 and ## p < 0.01 versus that of MB salt alone (c), M alone (d), or B alone (e).