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. 2016 Mar 30;2016:6570671. doi: 10.1155/2016/6570671

Figure 1.

Figure 1

Extracellular calcium ([Ca2+]o) promoted pBMSCs proliferation and CaSR expression. (a) Effect of various [Ca2+]o (1, 2, 4, and 6 mM) on the proliferation of pBMSCs (n = 8). (b) Effect of 4 mM [Ca2+]o on the mRNA expression of CaSR in pBMSCs after 5-day culture. (c) Western blot analysis of CaSR in pBMSCs after 5-day culture. β-actin was used as loading control. (d) Mean ± SEM of immunoblotting bands of CaSR; the intensities of the bands were expressed as the arbitrary units (n = 3). ∗ P < 0.05, ∗∗ P < 0.01, and ∗∗∗ P < 0.001 versus 1 mM [Ca2+]o group (control).