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. 2016 Mar 30;2016:6570671. doi: 10.1155/2016/6570671

Figure 5.

Figure 5

Inhibition of ERK pathway abolished [Ca2+]o-induced proliferation of pBMSCs. (a) Effects of U0126 (1 μM), an inhibitor of MEK, on the proliferation of pBMSCs after 5-day incubation (n = 8). (b) The mRNA expression levels of cyclins (cyclin A2, cyclin D3, and cyclin E2), PCNA, and p21 in response to 4 mM [Ca2+]o and/or 1 μM U0126. (c) Western blot analysis of cyclin D1, p21, phosphor-ERK (p-ERK), and ERK in pBMSCs after 5-day culture. β-actin was used as loading control. (d) Mean ± SEM of immunoblotting bands of cyclin D1, p21, and p-ERK/ERK. The intensities of the bands were expressed as the arbitrary units (n = 4). ∗ P < 0.05 and ∗∗∗ P < 0.001 versus 1 mM [Ca2+]o group (control); # P < 0.05 and ### P < 0.001 versus 4 mM [Ca2+]o group.