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Oligonucleotide primer sequences and amplicon length
| mRNA | Primer sequences (5’ … 3’) | Length (bp) | |
|---|---|---|---|
| α-SMA (mouse) | Sense | CATGTGCTACCCTTAACTTG | 170 |
| Anti-sense | CTGTTGAAGTGATTGATGCC | ||
| TGF-β1 (mouse) | Sense | GCAACATGTGGAACTCTACC | 149 |
| Anti-sense | GTATTCCGTCTCCTTGGTTC | ||
| TNF-α (mouse) | Sense | GTCGTAGCAAACCACCAAG | 145 |
| Anti-sense | AGAGAACCTGGGAGTAGAC | ||
| IL-1β (mouse) | Sense | TGTTTTCCTCCTTGCCTCTG | 135 |
| Anti-sense | ACACGTTGACAGCTAGGTTC | ||
| β2M (mouse) | Sense | ACCCGCCTCACATTGAAATCC | 146 |
| Anti-sense | GGCGTATGTATCAGTCTCAGTG |
